Volume 7, Issue 3 (September 2022)
J Environ Health Sustain Dev 2022, 7(3): 1727-1732 |
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Abdolahnejad A, Pourakbar M, Raeghi S, Mohammadi A, Ranjbar B, Behnami A. Experimental Protocol for Detecting SARS-CoV-2 in Screenings and Grit Samples of Wastewater Treatment Plants. J Environ Health Sustain Dev 2022; 7 (3) :1727-1732
URL: http://jehsd.ssu.ac.ir/article-1-456-en.html
URL: http://jehsd.ssu.ac.ir/article-1-456-en.html
Ali Abdolahnejad 
, Mojtaba Pourakbar , Saber Raeghi , Amir Mohammadi , Behzad Ranjbar , Ali Behnami *
, Mojtaba Pourakbar , Saber Raeghi , Amir Mohammadi , Behzad Ranjbar , Ali Behnami *
Department of Environmental Health Engineering, Maragheh University of Medical Sciences, Maragheh, Iran.
Abstract: (868 Views)
Introduction: Although various liquid, solid, and gaseous streams of wastewater treatment plants (WWTPs) have been analyzed in many studies for the presence of SARS-CoV-2 RNA, no study was found to sample and detect SARS-CoV-2 RNA in screenings and grit samples separated from primary treatment units of WWTP. Hence, this study aims to provide an experimental protocol for sampling and extracting SARS-CoV-2 RNA from screenings and grits separated from WWTPs.
Materials and Methods: First, sampling was conducted to extract SARS-CoV-2 RNA from screenings and grit samples. After sample processing and viral RNA extraction, SARS-CoV-2 RNA detection was performed by one-step reverse transcription quantitative polymerase chain reaction (RT-qPCR).
Results: Based on the results, SARS-CoV-2 RNA was successfully extracted from screenings and grit samples of the studied WWTP with concentrations of (1.54 –3.9 × 104) and (0.8 – 2.3 × 104) genomic copies/L, respectively.
Conclusion: Considering the successfully isolation and detection of SARS-CoV-2 viral RNA in solid phase samples of WWTP, this method can be applied for extracting SARS-CoV-2 RNA and maybe other viruses from the screenings and grit samples of WWTPs in related studies.
Materials and Methods: First, sampling was conducted to extract SARS-CoV-2 RNA from screenings and grit samples. After sample processing and viral RNA extraction, SARS-CoV-2 RNA detection was performed by one-step reverse transcription quantitative polymerase chain reaction (RT-qPCR).
Results: Based on the results, SARS-CoV-2 RNA was successfully extracted from screenings and grit samples of the studied WWTP with concentrations of (1.54 –3.9 × 104) and (0.8 – 2.3 × 104) genomic copies/L, respectively.
Conclusion: Considering the successfully isolation and detection of SARS-CoV-2 viral RNA in solid phase samples of WWTP, this method can be applied for extracting SARS-CoV-2 RNA and maybe other viruses from the screenings and grit samples of WWTPs in related studies.
Type of Study: Original articles |
Subject:
Water quality and wastewater treatment and reuse
Received: 2022/06/23 | Accepted: 2022/07/10 | Published: 2022/09/30
Received: 2022/06/23 | Accepted: 2022/07/10 | Published: 2022/09/30
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