en Environmental Health, Sciences, and Engineering Original articles <div style="text-align: justify;"><span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:115%"><span style="font-family:Calibri,&quot;sans-serif&quot;"><b><i><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Introduction</span></span></span></i></b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">: The aim of this study is the isolation and primary identification of isolated bioaerosols sampled from different locations, as well as determining their antibiotic resistance profile for the selected bacteria. </span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:115%"><span style="text-autospace:none"><span style="font-family:Calibri,&quot;sans-serif&quot;"><b><i><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Materials and Methods</span></span></span></i></b><i><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">:<b> </b></span></span></span></i><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">The air samples were collected from 35 stations in 17 buildings (bank, </span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">public, healthcare and industrial</span></span></span><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">). An air-sampling pump (Flite3-SKC) with a flow rate of 14.1 l/min was used for five minutes at the respiratory height. The total bacterial count and primary identification were carried out, then the antibiotic susceptibility tests for ten selected antibiotics were conducted by the disk diffusion method.<i>&nbsp; </i></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:115%"><span style="text-autospace:none"><span style="font-family:Calibri,&quot;sans-serif&quot;"><b><i><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Results</span></span></span></i></b><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">: The bacterial population varied at a range of 128-5503 CFU/m³, and the fruit and vegetable bazaar of Zanjan showed the highest population of bioaerosols. The bioaerosols population among industrial samples was high. Most isolates were determined as gram-positive Cocci (> 70%) and <i>Bacillus</i> spp, respectively. The most antibiotic-resistant bacteria were related to manufacturing activities, showing resistance to Chloramphenicol and Trimethoprim/ Sulfamethoxazole (72%). </span></span></span></span></span></span></span></span></span><br> <b><i><span style="font-size:10.0pt"><span style="line-height:107%"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">Conclusion</span></span></span></i></b><span style="font-size:10.0pt"><span style="line-height:107%"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">: The type of activity affects the bacterial population and antibiotic resistance in industrial uses. The increase of multi-drug resistant bacteria, especially in medical settings, is known as a community health challenge and needs more attention.</span></span></span></div> Air Pollution, Antibiotic, Resistant, Bioaerosols, Zanjan City. 1951 1961 http://jehsd.ssu.ac.ir/browse.php?a_code=A-10-616-1&slc_lang=en&sid=1 Arezoo Tavakoli a_tavakoli2003@yahoo.com 0000-0001-7746-2156 Yes Department of Nursing, Islamic Azad University, Eghlid branch, Fars, Iran. Azadeh Tavakoli atavakoli@znu.ac.ir 0000-0002-1179-8105 No Department of Civil Engineering, University of Zanjan, Zanjan, Iran. Fatemeh Karimi f.karimi123@gmail.com 0000-0003-4635-9855 No Department of Science, University of Zanjan, Zanjan, Iran.