en Environmental Health, Sciences, and Engineering Original articles <div style="text-align: justify;"><strong><em>Introduction:</em></strong> This study aimed to assess a good protocol for the contamination indexes, concentration, spatial analysis, and source identification of toxic metals in top soils.<br> <strong><em>Materials and Methods:</em></strong> In the first step, samples were taken from top soil (30 cm) and the metals were extracted and detected with ICP-AES. In the second step, Enrichment Factor, Geoaccumulation Index, and Contamination Factor of metals were calculated to determine soil contamination degree. Furthermore, the principal component analysis and correlation between metals were conducted for source identification.<br> <strong><em>Results:</em></strong> Spatial analysis, as an important section of the present protocol, was performed using Arc GIS, kriging, and Moran&#39;s I models. As results of Moran&#39;s I model showed, distribution pattern for Fe, As, Cd, Cu, Ni, Pb, and Zn were random (z-scores ranged from -1.17 to 1.09), indicatingthat these elements could be emitted from different potential sources. In Moran&#39;s model, spatial autocorrelation of each pollutant could be measured based on its value and location.<br> <strong><em>Conclusion:</em></strong> The finding of this protocol can be used for extraction of contamination indexes, concentration, spatial analysis, and source identification of toxic metals in top soils.</div> Environmental Pollution, Metals, Heavy, Spatial Analysis, Moran's model. 1155 1161 http://jehsd.ssu.ac.ir/browse.php?a_code=A-10-173-1&slc_lang=en&sid=1 Amir Mohammadi mohammadiurm@gmail.com 0000-0003-3932-0688 Yes Department of Public Health, Environmental Health Engineering, Maragheh University of Medical Sciences, Maragheh, Iran. Sepideh Nemati Mansour nemati.sepid@gmail.com 0000-0002-4187-0438 No Department of Environmental Health Engineering, School of Public Health, Tabriz University of Medical Sciences, Tabriz, Iran. Maryam Faraji m_faraji28@yahoo.com 0000-0002-7220-4438 No Environmental Health Engineering Research Center, Kerman University of Medical Sciences, Kerman, Iran Ali Abdolahnejad abdolahnejad.a@gmail.com 0000-0003-0193-7690 No Department of Public Health, Environmental Health Engineering, Maragheh University of Medical Sciences, Maragheh, Iran. Ali Toolabi 0000-0003-4573-6807 No Department of Environmental Health Engineering, School of Public Health, Bam University of Medical Sciences, Bam, Iran. Moahammad Miri m_miri87@yahoo.com 0000-0002-9424-6487 No Non-communicable Disease Research Center, Department of Environmental Health Engineering, Sabzevar University of Medical Sciences, Sabzevar, Iran.