en Environmental Health, Sciences, and Engineering Original articles <div style="text-align: justify;"><strong><em>Introduction:</em></strong> Heavy metal contamination has necessitated a less expensive and non-destructive clean-up technique such as mycoremediation. This study aimed to isolate, identify, and evaluate the tolerance of fungi species in different concentrations of heavy metals for their potential use in bioremediation.<br> <strong><em>Materials and Methods:</em></strong> Fungi were isolated by serial dilution and spread plate techniques from gold tailings and their tolerance to different concentrations of As, Cd, Cr, Cu, Pb, Zn (as potential bioremediation candidates) was evaluated. Fungal radial growths were recorded daily over a 14 days&rsquo; incubation period to establish their tolerance levels using the Tolerance Index.<br> <strong><em>Results:</em></strong> Five isolated fungi species belonged to the genera <em>Aspergillus</em>,<em> Trichophyton</em>,<em> Rhizopus</em>,<em> Trametes</em>, and <em>Trichoderma</em>. Except for <em>Trichophyton rubrum</em>, the other fungi species were tolerant to all Cr concentrations (0 &ndash; 100ppm), but no significant difference was observed in mycelia growth compared to their controls. With high tolerance index ranging from 0.91 to 1.02, <em>Trichoderma viride</em> and<em> Rhizopus oryzae </em>were tolerant to all Cu concentrations (0 &ndash; 125ppm). High tolerance was exhibited by <em>Trametes versicolor </em>to Cd at 25 and 50 ppm, and by <em>Rhizopus oryzae </em>at 25 ppm, but no significant difference was found in mycelia growth. <em>Rhizopus oryzae </em>tolerated all As and Pb concentrations with high tolerance index ranging from 0.81 to 1.00. It also tolerated&nbsp;&nbsp; Zn at 200-600ppm concentration with a tolerance index from 0.80 to 0.91.<br> <strong><em>Conclusion:</em> </strong>The selective nature of these fungi species for specific heavy metal tolerance indicates their potential for selective use as effective bioremediative clean up agent of heavy metals contaminated sites.</div> Contamination, Bioremediation, Fungi, Heavy Metals, Tolerance, Ghana. 1231 1242 http://jehsd.ssu.ac.ir/browse.php?a_code=A-10-248-1&slc_lang=en&sid=1 Emmanuel Sey immasey@gmail.com 0000-0002-8845-0297 Yes Department of Materials Engineering, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. Ebenezer J. D. Belford ejdbelford.sci@knust.edu.gh 0000-0002-4407-3880 No Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.